Vacuum would not make it miss in only one cylinder. As Vile stated, a compression test in all cylinders would show if there is any loss of pressure (due to head gasket or broken rings, cracked head, etc) in any one cylinder.
You said the spark plug was good, did you change it out and retrest? I would put a completely new plug in it and wire from distributor. Check out the distributor cap and ensure the the little nipple for that cylinder is ok....just replace the cap anyway.
SgtDel
aka "Andy"
www.delbridge.net
1979 Big 10 383 stroker
1991 Blu 'Burb 3/4 ton 4x4